Path: utzoo!utgpu!watserv1!watmath!att!emory!ox.com!caen!zaphod.mps.ohio-state.edu!wuarchive!uunet!stanford.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mouse mRNA for idiotypic anti-NP IgG(1) heavy chain V-D-J Message-ID: Date: 27 May 91 12:42:54 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 51 Approved: lear@genbank.bio.net Checksum: 51809 3 LOCUS MUSIGNP4 411 bp ss-mRNA ROD 27-MAY-1991 DEFINITION Mouse mRNA for idiotypic anti-NP IgG(1) heavy chain V-D-J (hybridoma P.10.15.1/group VI) ACCESSION X02565 KEYWORDS diversity region; gamma-immunoglobulin; immunoglobulin; immunoglobulin heavy chain; joining region; signal peptide; variable region. SOURCE Mus musculus RNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 411) AUTHORS Boersch-Supan,M.E., Agarwal,S., White-Scharf,M.E. and Imanishi-Kari,T. TITLE Heavy chain variable region Multiple gene segments encode anti-4-(hydroxy-3-nitropheny)acetyl idiotypic antibodies JOURNAL J. Exp. Med. 161, 1272-1292 (1985) STANDARD full automatic COMMENT Serological analysis of hybridomaproteins resulting from the immune response to the hapten NP reveals NP(a) idiotypes expressed by Balb/C mice and NP(b) idiotypes expressed by C57 BL/6 mice. The NP(b) family of antibodies consists of 6 subgroups (I-VI) with I-IV sharing more determinants than V and VI which appear quite distinct. From EMBL entry MMIGNP4; dated 16-JAN-1991. FEATURES Location/Qualifiers CDS join(58..411) /product="idiotypic anti-NP IgG(1) heavy chain V-D- J" /codon_start=58 sig_peptide 1..57 misc_feature 58..351 /note="variable region (aa 1-98)" misc_feature 148..162 /note="CDR I" misc_feature 205..255 /note="CDR II" misc_feature 352..366 /note="D-region (aa 99-103)" misc_feature 367..>411 /note="J-region (aa 104-118)" BASE COUNT 100 a 104 c 111 g 96 t ORIGIN 1 atgaaatgca gctgggtcat gttcttcctg atggcagtgg ttacaggggt caattcagag 61 gttcagctgc agcagtctgt ggcagagctt gtgaggccag gggcctcagt caggttgtcc 121 tgcacagctt ctggcttcaa cattaaaaac acctatatgc actgggtgaa gcagaggcct 181 gaacaaggcc tggagtggat tggaaggatt gatcctgcga atggtaatac taaatatgcc 241 ccgaagttcc aggtcaaggc cactataaca gcagacacat cctccaacac agcctacctg 301 cagctcagca gcctgacatc tgaggacact gccatctatt actgtgcccc ttattactac 361 ggggggtact tcgatgtctg gggcgcaggg accacggtca ctgtttcctc a //