Path: utzoo!utgpu!watserv1!watmath!att!emory!swrinde!zaphod.mps.ohio-state.edu!wuarchive!uunet!stanford.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mouse germline immunoglobulin V(H)II pseudogene H24 Message-ID: Date: 27 May 91 12:43:09 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 61 Approved: lear@genbank.bio.net Checksum: 50829 3 LOCUS MUSIGVH7 676 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse germline immunoglobulin V(H)II pseudogene H24 ACCESSION X02460 KEYWORDS enhancer; germline; immunoglobulin; immunoglobulin heavy chain; pseudogene; signal peptide; variable region; variable region subgroup VH-II. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 676) AUTHORS Schiff,C., Milili,M. and Fougereau,M. TITLE Functional and pseudogenes are similarly organized and may equally contribute to the extensive antibody diversity of the IgVHII family JOURNAL EMBO J. 4, 1225-1230 (1985) STANDARD full automatic COMMENT From EMBL entry MMIGVH7; dated 16-JAN-1991. FEATURES Location/Qualifiers sig_peptide join(147..192,276..286) CDS join(147..192,276..580) /note="(aa 1-94, kabat numbering)" /product="H24 variable region" /codon_start=147 misc_feature 40..49 /note="sequence homologous to SV40 enhancer" promoter 67..72 /note="pot. TATA-box" precursor_RNA 95..>94 /note="pot. H24 primary transcript" mRNA 95..194 /note="pot. H24 mRNA; exon 1" misc_RNA 95..95 /note="put. CAP site" intron 193..275 /note="intron I" mRNA 276..>275 /note="H24 mRNA; exon 2" mat_peptide 287..580 /note="(aa 1-94, kabat numbering)" /product="H24 variable region" misc_feature 530..532 /note="in frame stop codon of pseudogene" misc_feature 581..587 /note="recombination signal" misc_feature 611..619 /note="recombination signal" BASE COUNT 174 a 168 c 168 g 166 t ORIGIN 1 tggactagat ccttactaag taatgcactg ctcatgaata tgcaaatcac gcaagtcttt 61 ggcagtaaat actgggatgt ccacaccctg aaaacaacct aagatcagtg tcctctccac 121 agtccctgaa cacactgact ttcaccatgg aatggagcgg ggtctttatc tttctcctgt 181 cagtaactgc aggtaagggg ctccccattt ccaaatctga agatgataca gggcctgagg 241 tgacaatgcc aaccactctg cctttctctc cacaggtgtc cactcgcagg tccagctgca 301 gcagtctgga gctgagctgg taaggcctgg gacttcagtg aagaagtcct gcaaggtttc 361 tggatacacc ttcgctaact actggatagg ttgggtaaag cagaggcctg gacatggcct 421 tgagtggatt ggagatattt accctggaga cggtgttact aactacaatg agaagttcaa 481 gggcaaggcc acactgactg cagacaaatc ctccagcaca gcctacatgt agctcagcag 541 cctgacatct gaggactctg cggtctatta ctgttcgaga cacagtgttg caaccacatc 601 ctgagtgtgt cagaaaccct ggaggagtag caaactgccc tgggtctgag ttgtctcagt 661 gaaggtttct ttgtag //