Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mouse nonproductive mRNA for T-cell receptor alpha chain (TA80) Message-ID: Date: 27 May 91 12:43:59 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 46 Approved: lear@genbank.bio.net Checksum: 56683 3 LOCUS MUSTCRA80 293 bp ss-mRNA ROD 27-MAY-1991 DEFINITION Mouse nonproductive mRNA for T-cell receptor alpha chain (TA80) ACCESSION X02939 KEYWORDS T-cell receptor; T-cell receptor alpha; constant region; joining region; variable region. SOURCE Mus musculus RNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 293) AUTHORS Arden,B., Klotz,J.L., Siu,G. and Hood,L. TITLE Diversity and structure of genes of the alpha family of mouse T-cell antigen receptor JOURNAL Nature 316, 783-787 (1985) STANDARD full automatic COMMENT *source: strain=C57Bl/Ka; clone=TA80; The substitution changing the put. important cys residue and the frameshifting V-J rearrangement indicate that the sequence is probably a transcript derived from rearrangement of pseudogenes. The 3' end of V(alpha) segment was determined referring to germline V(alpha) 5H gene segment (see X02833). The 5' end of J(alpha) segment was determined in comparison to corresponding germline segment (see X02859). From EMBL entry MMTCRA80; dated 02-APR-1988. FEATURES Location/Qualifiers CDS <1..219 /note="put. V(alpha) 5 segment (put. aa 20-93)" /codon_start=1 misc_feature 7..9 /note="put. substitution site changing cys to ala" misc_feature 220..224 /note="frameshifting junctional region" CDS 225..282 /note="J(alpha) segment" /codon_start=225 CDS 283..>293 /note="C(alpha) segment" /codon_start=283 BASE COUNT 90 a 78 c 64 g 61 t ORIGIN 1 ataacggcac cctacacaga ccctaacagt tattacttct tctggtacaa gcaagagccg 61 ggggcaagtc ttcagttgct tatgaaggtt ttctcaagta cggaaataaa cgaaggacaa 121 ggattcactg tcctactgaa caagaaagac aaacgactct ctctgaacct cacagctgcc 181 catcctgggg actcagccgc gtacttctgc gcagtcagtg aaagacagaa ggtgcagata 241 gactcacctt tgggaaagga actcagctga tcatccagcc ctacatccag aac //