Path: utzoo!utgpu!watserv1!watmath!att!emory!swrinde!zaphod.mps.ohio-state.edu!magnus.acs.ohio-state.edu!usenet.ins.cwru.edu!agate!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Mouse mRNA for T-cell specific gamma chain V(10.8A)-J-C(10.5)part. Message-ID: Date: 27 May 91 12:44:20 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 53 Approved: lear@genbank.bio.net Checksum: 64531 3 LOCUS MUSTCRG1P 508 bp ss-mRNA ROD 27-MAY-1991 DEFINITION Mouse mRNA for T-cell specific gamma chain V(10.8A)-J-C(10.5)part. clone 8/10-2 gamma 1.1; nonproductive transcript ACCESSION X03801 KEYWORDS T-cell receptor; T-cell receptor gamma; constant region; joining region; signal peptide; variable region. SOURCE Mus musculus RNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 508) AUTHORS Iwamoto,A., Rupp,F., Ohashi,P.S., Walker,C.L., Pircher,H., Joho,R., Hengartner,H. and Mak,T.W. TITLE T-cell-specific gamma-genes in C57BL/10 mice - Sequence and expression of new constant and variable region genes JOURNAL J. Exp. Med. 163, 1203-1212 (1986) STANDARD full automatic COMMENT The 8/10-2 gamma1.1 sequence is identical to pHDS4/203 (see /) except for two missing nucleotides between pos.447-448 causing a translational frameshift. Presumably the 8/10-2 gamma1.1 sequence arose frome a nonproductive gene rearrangement involving the same V(gamma 10.8) and J-C(gamma 10.5) gene segments which gave rise to the pot. functional pHDS4/203 message. From EMBL entry MMTCRG1P; dated 18-NOV-1986. FEATURES Location/Qualifiers CDS 88..141 /note="signal peptide" /codon_start=88 CDS 142..438 /note="variable region" /codon_start=142 misc_feature 439..441 /note="premature termination codon" CDS 439..497 /note="joining region" /codon_start=439 CDS 498..>508 /note="constant region" /codon_start=498 BASE COUNT 159 a 119 c 105 g 125 t ORIGIN 1 tagcacagtc ccaccctcca gctggtgacc tgaaattcca gcctgcagag cacttcctgc 61 ctccctgtga agcagtccaa ccttgggatg ctgctcctga gatggttcac ctcctgctgc 121 ctctgggttt ttgggcttgg gcagctggag caaactgaat tatcggtcac cagagagaca 181 gatgagaatg tgcaaatatc ctgtatagtt tatcttccat atttctccaa cacagctata 241 cattggtacc ggcaaaaaac aaatcaacag tttgagtatc taatatatgt cgcaacaaac 301 tacaatcaac gacccttagg agggaagcac aaaaaaattg aagcaagtaa agattttaaa 361 agttctacct caaccttgga aataaattac ttgaagaaag aagatgaagc cacctactac 421 tgtgcagtct ggatgagata gctcgggctt tcacaaggta tttgcagaag gaacaaagct 481 catagtaatt ccctccgaca aaaggctt //