Path: utzoo!utgpu!watserv1!watmath!att!news.cs.indiana.edu!spool.mu.edu!think.com!zaphod.mps.ohio-state.edu!wuarchive!uunet!bionet!will From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Human parainfluenza virus type 3 (PF3), fusion protein (F) mRNA, Message-ID: Date: 28 May 91 18:44:22 GMT Article-I.D.: genbank.May.28.11.44.21.1991.8563 Sender: will@genbank.bio.net Distribution: bionet Lines: 70 Approved: lear@genbank.bio.net Checksum: 21888 5 LOCUS SNDMPF3F 1845 bp ss-mRNA VRL 28-MAY-1991 DEFINITION Human parainfluenza virus type 3 (PF3), fusion protein (F) mRNA, complete cds. ACCESSION D00016 N00016 KEYWORDS Paramyxovirus; fusion protein F; parainfluenza virus; parainfluenza virus type 3. SOURCE Parainfluenza virus type 3 RNA. ORGANISM Parainfluenza virus type 3 Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses; Paramyxoviridae; Paramyxovirus. REFERENCE 1 (bases 1 to 1845) AUTHORS Spriggs,M.K., Olmsted,R.A., Venkatesan,S., Coligan,J.E. and Collins,P.L. TITLE Fusion glycoprotein of human parainfluenza virus type 3: Nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses JOURNAL Virology 152, 241-251 (1986) STANDARD full automatic COMMENT SWISS-PROT; P06828; VGLF$PI3H4. The fusion (F) mRNA contains 1845 nucleotides, exclusive of poly(A), has an unusually long (193-nucleotide) 5'nontranslated region, and encodes an unprocessed Fo protein of 539 amino acids. To establish the authenticity of the cDNA sequence, the F gene was sequenced directly, in its entirety by dideoxynucleotide sequencing of vRNA. This confirmed the cDNA sequence in general, but also identified differences between the cDNAs and vRNA at eight nucleotide positions (position 494, 614, 652, 1093, 1564, 1574, 1588, 1783). These are thought to represent variability introduced during PF3 RNA synthesis (reported in [1]). From EMBL entry PAMPF3F; dated 26-APR-1990. FEATURES Location/Qualifiers CDS 194..1813 /note="Fusion protein (F)" /codon_start=194 BASE COUNT 736 a 332 c 323 g 454 t ORIGIN 1 aggacaaaag aagtcaatac caacaactat tagcagccac actcgctgga acaagaaaga 61 agggataaaa aaagtttaac agaagaaaca aaaacaaaaa gcacagaaca ccagaacaac 121 aagatcaaaa cacccaaccc actcaaaacg aaaatctcaa aagagattgg caacacaaca 181 aacactgaac atcatgccaa cctcaatact gctaattatt acaaccatga ttatggcatc 241 tttctgccaa atagatatca caaaactaca gcatgtaggt gtattggtta acagttccaa 301 agggatgaag atatcacaaa actttgaaac aagatatcta attttgagcc tcataccaaa 361 aatagaagat tctaactctt gtggtgacca acagatcaag caatacaaga ggttattgga 421 tagactgatc attcctttat atgatggatt aagattacag aaggatgtga tagtgtccaa 481 tcaagaatcc aatgaaaaca ctgaccccag aacaaaacga ttctttggag gggtaattgg 541 aactattgct ctgggagtgg caacctcagc acaaattaca gcggcagttg ctctggttga 601 agccaagcag gcaagatcag acattgaaaa actcaaggaa gcaatcaggg acacaaacaa 661 agcagtgcag tcagtccaga gctccatagg aaatttgata gtagcaatta aatcggtcca 721 ggattatgtc aacaaagaaa tcgtgccatc aattgcgaga ttaggttgtg aagcagcagg 781 acttcagtta ggaattgcat taacacagca ttactcagaa ttaacaaaca tattcggtga 841 taacatagga tcgttacaag aaaaagggat aaaattacaa ggtatagcat cattataccg 901 cacaaatatc acagagatat tcacaacatc aacagttgat aaatatgata tttatgatct 961 attatttaca gaatcaataa aggtgagagt tatagatgtt gacttgaatg attactcgat 1021 cgccctccaa gtcagactcc ctttattaac tagactgctg aacacccaga tttacagagt 1081 agattccata tcatataaca tccaaaacag agaatggtat atccctcttc ccagccacat 1141 catgacaaaa ggggcatttc taggtggagc agatgtcaaa gaatgtatag aagcattcag 1201 cagttatata tgcccttctg atccaggatt tgtactaaac catgaaatgg agagctgttt 1261 atcaggaaac atatcccaat gtccaagaac cgtggttaaa tcagacattg ttccaagata 1321 tgcatttgtc aatggaggag tggttgcaaa ttgtataaca accacatgta catgcaacgg 1381 tatcggtaat agaatcaatc aaccacctga tcaaggagta aaaattataa cacataaaga 1441 atgtaataca ataggtatca acggaatgct gttcaataca aataaagaag gaactcttgc 1501 attttacaca ccaaatgata taacattaaa caattctgtt gcacttgatc caattgacat 1561 atcaatcgag ctcaataagg ccaaatcaga tctagaagag tcaaaagaat ggataagaag 1621 gtcaaatcaa aaactagatt ccattggaaa ttggcatcaa tctagcacca caatcataat 1681 tgttttgata atgataatta tattgtttat aattaatgta acgataatta taattgcagt 1741 taagtattac agaattcaaa agagaaatcg agtggatcaa aatgataaac catatgtatt 1801 aacaaacaaa tgacagatct atagatcatt agatattaaa attat //