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From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human germline kappa-immunoglobulin variable region (V-kappa-I)
Message-ID: <May.30.05.12.59.1991.24382@genbank.bio.net>
Date: 30 May 91 12:13:00 GMT
Sender: root@genbank.bio.net
Distribution: bionet
Lines: 76
Approved: lear@genbank.bio.net
Checksum: 07307     4

LOCUS       HUMVKI       1394 bp ds-DNA             PRI       30-MAY-1991
DEFINITION  Human germline kappa-immunoglobulin variable region (V-kappa-I)
            gene (Va gene)
ACCESSION   X17262
KEYWORDS    immunoglobulin; immunoglobulin light chain; kappa immunoglobulin;
            kappa light chain.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Pech,M., Smola,H., Pohlenz,H.D., Straubinger,B., Gerl,R. and
            Zachau,H.G.
  TITLE     A large section of the gene locus encoding human immunoglobulin
            variable regions of the kappa type is duplicated.
  JOURNAL   J. Mol. Biol. 183, 291-299 (1985)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Klobeck,H.G.
  JOURNAL   Unpublished (1989)
  STANDARD  full automatic
COMMENT     *source: developmental stage=germline; tissue=placenta; *source:
            cell line=placenta St; **map: chromosome=2p12; See <X17262-X17265>
            for other V kappa immunoglobulin genes.
            
            From EMBL    entry HSVKI;  dated 30-JUL-1990.
FEATURES             Location/Qualifiers
     mRNA            <185..184
                     /note="exon 1"
     misc_feature    2..13
                     /note="(put.) pd regulatory element"
     misc_feature    33..42
                     /note="(put.) dc regulatory element"
     promoter        75..82
                     /note="TATA-box"
     CDS             130..184
                     /note="leader peptide (AA 1-18) (184 is 1st base in
                     codon)"
                     /codon_start=130
     intron          185..310
                     /note="intron I"
     mRNA            311..606
                     /note="exon 2"
     CDS             311..606
                     /note="leader peptide/variable region (AA 19-117) (311 is
                     2nd base in codon)"
                     /codon_start=311
     misc_feature    609..615
                     /note="(non canonical) heptamer recombination signal"
BASE COUNT      391 a    308 c    315 g    380 t
ORIGIN
        1 ctgcagctgt gtccagcctg cccatcctct gctcatttgc atattcccag aacacaacct
       61 cctgccctga agacttctta ataggctggt cacacttctt gcaggagtca gacccactca
      121 ggacacagca tggacatgag ggtccccgct cagctcctgg ggcttctgct gctctggctc
      181 ccaggtaagg aaggagaaca ctaacagttt actcagccca gggggctcag tacagcctgg
      241 ctattcaggg aaattctctt actacatgat taattgtgtg gaccatttgt gtttatgctt
      301 ccaatctcag gtgccagatg tgccatccag ttgacccagt ctccatcctc cctgtctgca
      361 tctgtaggag acagagtcac catcacttgc cgggcaagtc agggcattag cagtgcttta
      421 gcctggtatc agcagaaacc agggaaagct cctaagctcc tgatctatga tgcctccagt
      481 ttggaaagtg gggtcccatc aaggttcagc ggcagtggat ctgggacaga tttcactctc
      541 accatcagca gcctgcagcc tgaagatttt gcaacttatt actgtcaaca gtttaataat
      601 taccctcaca tagtgttaca aacccgaaca taaaccccca gggaagcaga tgtgtgagac
      661 tgggctgccc cagctgcttc tcctgatgcc tccattggct gagagtgttc ctcagatgca
      721 gccacactct gatggtgttg gtagaggagg atatgagatc acctctgcat cccaatttct
      781 ttttcttttc tcagccccag ctgcacagac ataacaatgc ctctgctgat ttaataaaga
      841 tagagatcat gacacctgaa gagtctagtt tatggctttg gttagaattc atataacaga
      901 gaagaagcca ttatagatat tctaagcagg aatagtctta atagatagaa ttagagtcta
      961 aagtattgaa gtctaaataa aatgtacaga taaatttagt gttttatttg ctaagaaatt
     1021 tttgccaaat ggggcataca ggaaaactca atggtcttca atatgttgga agagcaaaga
     1081 gttttataaa aagggaaatt attacctatt gttctttgag aaattttgtt ggctgtagta
     1141 agggttggga gctggcaagc tcagactggt aagcagtggt ggtcaaactg aatcctagaa
     1201 ttatattaag ttatctcaga agttgtgggt aaatttgctt tcaggttaca ataagccaaa
     1261 gcagtgaagc ttgcagagaa ttttgttact gaaatgccag ggattcagta tagatcctgc
     1321 ggctcaccac acagaaagcc aatcactaag acaacaagtg ttgtcaaaga acaggcttta
     1381 atcaggtgct gcag
//