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From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human germline kappa-immunoglobulin variable region (V-kappa-I)
Message-ID: <May.30.05.13.11.1991.24423@genbank.bio.net>
Date: 30 May 91 12:13:11 GMT
Sender: root@genbank.bio.net
Distribution: bionet
Lines: 67
Approved: lear@genbank.bio.net
Checksum: 33352     4

LOCUS       HUMVKIB       885 bp ds-DNA             PRI       30-MAY-1991
DEFINITION  Human germline kappa-immunoglobulin variable region (V-kappa-I)
            gene (Vb gene)
ACCESSION   X17263
KEYWORDS    immunoglobulin; immunoglobulin light chain; kappa immunoglobulin;
            kappa light chain.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Pech,M., Smola,H., Pohlenz,H.D., Straubinger,B., Gerl,R. and
            Zachau,H.G.
  TITLE     A large section of the gene locus encoding human immunoglobulin
            variable regions of the kappa type is duplicated.
  JOURNAL   J. Mol. Biol. 183, 291-299 (1985)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 885)
  AUTHORS   Klobeck,H.G.
  JOURNAL   Unpublished (1989)
  STANDARD  full automatic
COMMENT     *source: developmental stage=germline; tissue=placenta; *source:
            cell line=placenta St; **map: chromosome=2p12; See <X17262-X17265>
            for other V kappa immunoglobulin genes.
            
            From EMBL    entry HSVKIB;  dated 30-JUL-1990.
FEATURES             Location/Qualifiers
     mRNA            <186..185
                     /note="exon 1"
     misc_feature    2..13
                     /note="(put.) pd regulatory element"
     misc_feature    34..43
                     /note="(put.) dc regulatory element"
     promoter        76..83
                     /note="TATA-box"
     CDS             131..185
                     /note="leader peptide (AA 1-18) (185 is 1st base in
                     codon)"
                     /codon_start=131
     intron          186..309
                     /note="intron I"
     mRNA            310..605
                     /note="exon 2"
     CDS             310..605
                     /note="leader peptide/variable region (AA 19-117) (310 is
                     2nd base in codon)"
                     /codon_start=310
     misc_feature    608..614
                     /note="(non canonical) heptamer recombination signal"
BASE COUNT      207 a    240 c    208 g    230 t
ORIGIN
        1 ctgcagctgc gcccagcctg ccccatcccc tgctcatttg catgttccca gagcacagtc
       61 tcctgacctg aagacttatt aacaggctga tcacaccctg tgcaggagtc agacccagtc
      121 aggacacagc atggacatga gggtccccgc tcagctcctg gggctcctgc tgctctggtt
      181 cccaggtaag aaaggagaac actaggatta tactcggtca gtgtgctgag tactgcttta
      241 ctattcaggg aacttctctt acagcatgat taattgtgtg gacatttgtt tttatgtttc
      301 caatctcagg ttccagatgc gacatccaga tgacccagtc tccatcttct gtgtctgcat
      361 ctgtaggaga cagagtcacc atcacttgtc gggcgagtca gggtattagc agctggttag
      421 cctggtatca gcagaaacca gggaaagccc ctaagctcct gatctatgct gcatccagtt
      481 tgcaaagtgg ggtcccatca aggttcagcg gcagtggatc tgggacagat ttcactctca
      541 ctatcagcag cctgcagcct gaagattttg caacttacta ttgtcaacag gctaacagtt
      601 tccctcccac agtgttacca acccgaacat aaacccccag ggaagcagat gtgtgaagct
      661 gggctgcccc agctgctcct cctgatgcct ccattggctg agagtgttgc tcagatgcag
      721 ccacactctg atggtgttgg tagaggggta cgtgaaatcg cctctgcacc ctaattcttt
      781 tctctttctc agccccaact gcacagacat agcaatgcat ctcctgattt gataaataca
      841 gagatcatga cacttgagga gtctagttta tggcttcagc ttgaa
//