Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!sol.ctr.columbia.edu!ucselx!bionet!root From: GenBank-Updates@genbank.bio.net Newsgroups: bionet.molbio.genbank.updates Subject: Streptomyces hygroscopicus, 2-phosphinomethylmalic acid synthase Message-ID: Date: 30 May 91 12:16:10 GMT Sender: root@genbank.bio.net Distribution: bionet Lines: 26 Approved: lear@genbank.bio.net Checksum: 46558 2 LOCUS STMPMMST 144 bp ds-DNA BCT 30-MAY-1991 DEFINITION Streptomyces hygroscopicus, 2-phosphinomethylmalic acid synthase gene, amino terminus. ACCESSION D00709 KEYWORDS 2-phosphinomethylmalic acid; 2-phosphinomethylmalic acid synthase. SOURCE Streptomyces hygroscopicus, strain SF-1293, chromosomal DNA, clone pMSB12, subclone pMW2. ORGANISM Streptomyces hygroscopicus Prokaryota; Bacteria; Firmicutes; Streptomycetaceae. REFERENCE 1 (bases 1 to 144) AUTHORS Shimotohono,K.W., Imai,S., Murakami,T. and Seto,H. TITLE Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase JOURNAL Agric. Biol. Chem. 54, 463-470 (1990) STANDARD full staff_entry FEATURES Location/Qualifiers CDS 54..>144 /note="2-phosphinomethylmalic acid synthase, amino terminus" BASE COUNT 28 a 57 c 38 g 21 t ORIGIN 84 bp upstream of ScaI site. 1 accggcctag accttgcacc cgtcgcttac cgcaccgcag ggagcgagcc ccgatgaccg 61 tccagaatcc tcaggaaccc gagtacttcc cggaggtctt cccccaagac gccttccccc 121 agtacgcctg ggacgagggc atgc //