Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!think.com!sdd.hp.com!wuarchive!uunet!nih-csl!helix.nih.gov!donnel From: donnel@helix.nih.gov (Donald A. Lehn) Newsgroups: bionet.molbio.methds-reagnts Subject: Re: Vacuum pumps for gel drying Message-ID: <1545@nih-csl.nih.gov> Date: 30 May 91 13:20:37 GMT References: <9105291140.AA01516@genbank.bio.net> Sender: news@nih-csl.nih.gov Organization: National Institutes of Health, Bethesda Lines: 52 In article <9105291140.AA01516@genbank.bio.net> PHILLIPSA@lars.afrc.ac.uk writes: ->I've never been in a laboratory which has a wholly satisfactory ->system for drying polyacrylamide and agarose gels. The BioRad gel ->dryers are fine, it's the vacuum pumps which present the problem. ->Most labs I've been in use in-line vapour traps to remove the corrosive ->solvents, but these don't seem to be very efficient and the vacuum pump ->has a limited life even with frequent oil changes. Our present system ->uses an Aquavac, which (as I understand it) is a water vacuum pump driven ->by high-pressure water. This is OK, but doesn't give the greatest vacuum ->and ours needs replacing anyway. ->To get to the point, I've come across literature for a pump made by ->Genevac, which uses a Cole pump system. This seems ideal (although not ->cheap), as it has few moving parts and is highly corrosion-resistant. ->Has anyone experience of this type of pump with corrosive vapours? ->Can someone recommend a better system? -> ->Thanks for your help -> ->Andy Phillips ->Long Ashton Research Station, Bristol, UK ->JANET: PHILLIPS@UK.AC.AFRC.LARS ->EARN/BITNET: PHILLIPSA@LARS.AFRC.AC.UK Regarding your input for information on the Genevac. We have a Genevac pump model number CVP100/2 in our lab here at the National Institutes of Health. Based on how well this pump works and the type of service we have had from the company I feel you would have to be a complete fool to believe that this product actually works. In the 18 months that we have had the pump it has actually worked for a total of not more than 116 hours. This is not because we do not use it but because the pump has broken down virtually every time we have tried to use it. The service from Genevac is even less efficient than their pump. The pump originally stopped working several hours after the company service rep came to the lab and set it up. It took more than 6 weeks before we could get the pump serviced. After the pump was serviced, a small hose broke on the pump broke and it took more than 2 months in order to get a replacement. Currently, the pump is once again down. We called Genevac several months ago in order to have it serviced and we are still waiting for the repair man. Based on my experience with Genevac and their poor service record, I would highly recommend against dealing with this company. If you need a reliable vacuum system, buy a traditional high quality vacuum pump and put on it the filter system sold by Svant. I have found that the Savant filter system is excellent in protecting the pump connected to a gel drier. Best regards, Donald A. Lehn National Institutes of Health Email: donnel@helix.nih.gov