Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!usc!elroy.jpl.nasa.gov!sdd.hp.com!caen!uwm.edu!bionet!har-rbu.mrc.ac.uk!MULTI-DS From: MULTI-DS@har-rbu.mrc.ac.uk Newsgroups: bionet.jobs Subject: (none) Message-ID: <9106031655.AA10746@genbank.bio.net> Date: 3 Jun 91 13:07:19 GMT Sender: daemon@genbank.bio.net Distribution: bionet Lines: 82 MRC RESEARCH STUDENTSHIP MOLECULAR GENETICS OR CELL & MOLECULAR BIOLOGY Applications are invited for a research studentship in one of the following fields: Molecular analysis of DNA repair and mutagenesis in human cells (Dr J Thacker); The majority of damage to DNA is repaired correctly by specific enzymes in cells, but this process can go wrong to give mutations. We are particularly interested in the formation of deletions in cells, since these are the major form of mutation induced by radiations in the environment. Deletion formation is being characterized by molecular analysis of breakpoints in genes of primary human cells, but we have also observed deletion formation in plasmid DNA molecules treated with human cell-free extracts. Extracts from a cell line derived from the human disorder ataxia-telangiectasia, characterized by radiosensitivity and cancer-proneness, yields a higher frequency of deletions than normal cell extracts. Chromosome imprinting (Dr Bruce Cattanach/Dr Jo Peters); Recent studies in the mouse have clearly established that maternal and paternal copies of certain genes, or chromosome regions, are not functionally equivalent. For example duplication of particular chromosomes from one parent with loss of the homologue from the other parent can cause embryonic lethality and problems with growth and viability. This phenomenon implies that parental chromosomes must be marked, or imprinted, as they pass through the parental germ lines so that they are distinguishable in the zygote. To date specific developmental anomaly or lethality has been associated with parental imbalance in each of 9 chromosome regions in the mouse and it is now clear that imprinting is a general mammalian phenomenon and is of considerable relevance to human medicine. The bulk of current evidence suggests that growth factors are implicated. The project will comprise expression studies aimed at identifying genes subject to imprinting, studying their effects during development and investigating the mechanism controlling the differential expression of maternal and paternal copies of genes. There are many links with research departments in Oxford which is 15 miles away. The studentship is available for 3 years from October 1991. Standard MRC stipend applies (#5,591 pa) plus up to #1,500 for laboratory duties. Please send applications, as quickly as possible, to the Personnel Officer, MRC Radiobiology Unit, Chilton, Didcot, Oxon OX11 0RD. Tel: 0235-834393 ext 235 for informal enquiries. MRC RADIOBIOLOGY UNIT, OXON MOLECULAR BIOLOGIST/GENETICIST A post is available in the Genetics Division to work with Dr M F Lyon in a small group of scientists investigating molecular genetics of the mouse. The work includes studies of mouse genetic mapping and the mouse t-complex, and encompasses varied projects and techniques in recombinant DNA technology, mouse genetics and developmental biology including transgenic mice. A wide range of mutants is available. The post is suitable for a post-doctoral scientist, but candidates with BSc and above will be considered; relevant experience is important. The post is available initially for one year, with the possibility of extension. Salary according to qualifications and experience. We are situated on the Harwell Laboratory site and offer excellent working conditions in pleasant rural surroundings, convenient for Oxford. Hostel accommodation is available. Informal enquiries welcome to Dr M F Lyon on 0235-834393. Please write or telephone for an application form to the Personnel Officer, MRC Radiobiology Unit, Chilton, Didcot, Oxon OX11 0RD. Tel: 0235-834882 (24 hour answering machine). The Council is an Equal Opportunities Employer.