Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!think.com!spool.mu.edu!uunet!ns-mx!vaxa.weeg.uiowa.edu!klautky
From: klautky@vaxa.weeg.uiowa.edu (BIOCHEMISTRY)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Analysis of plasmid after transfection?
Message-ID: <6364@ns-mx.uiowa.edu>
Date: 6 Jun 91 14:13:58 GMT
Article-I.D.: ns-mx.6364
Sender: news@ns-mx.uiowa.edu
Reply-To: klautky@vaxa.weeg.uiowa.edu
Organization: Weeg Computing Center, University of Iowa, Iowa City, IA, USA
Lines: 12
News-Software: VAX/VMS VNEWS 1.3-4


Dear Net Readers,

    Does anyone know of a protocol (or direct me to a reference) for the
analysis of plasmid DNA after transfection?

    I am interested in a method(s) that can quantitate the plasmid transfected
and determine intactness with respect to supercoiling.  Obviously, a
Southern can be used to check the latter but it is the former that I am
unsure of.

                Thanks in advance,                 Stephen Klautky