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From: ALSOBROOK@yalemed.bitnet
Newsgroups: bionet.molbio.methds-reagnts
Subject: quantitate plasmids
Message-ID: <4A927DAD8000D985@YALEMED.BITNET>
Date: 7 Jun 91 17:21:00 GMT
Sender: daemon@genbank.bio.net
Distribution: bionet
Lines: 5

Well, if you're going to do a Southern, one way to quantitate the plasmid
is to use known quantities of the plasmid on the same blot. After hybrid-
ization, you can use the known plasmid signals as a calibration curve.
Even without a densitometer, this will give a fairly accurate estimate of
how many plasmid copies are present.