Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!thunder.mcrcim.mcgill.edu!snorkelwacker.mit.edu!usc!zaphod.mps.ohio-state.edu!caen!spool.mu.edu!cs.umn.edu!talon.UCS.ORST.EDU!orstcs! From: demelerb@bionette.cgrb.orst.edu (Borries Demeler - Biochem) Newsgroups: bionet.molbio.methds-reagnts Subject: E.coli host cells (rec deficient) Message-ID: <1991Jun12.192034.6348@lynx.CS.ORST.EDU> Date: 12 Jun 91 19:20:34 GMT Sender: @lynx.CS.ORST.EDU Organization: Oregon State University, Corvallis Lines: 8 Nntp-Posting-Host: bionette.cgrb.orst.edu We are looking for an E. coli hostcell which prevents recombination events in plasmids containing many repeats of the same gene. We tried several recA- strains, but recombination still occured. Any suggestions of potentially useful hostcells would be appreciated. Please e-mail your help to demelerb@bionette.cgrb.orst.edu Thank you very much, -Borries