Path: utzoo!utgpu!news-server.csri.toronto.edu!bonnie.concordia.ca!uunet!cis.ohio-state.edu!sei.cmu.edu!fs7.ece.cmu.edu!o.gp.cs.cmu.edu!andrew.cmu.edu!ew2f+ From: ew2f+@andrew.cmu.edu (Elaine M. Weidenhammer) Newsgroups: bionet.molbio.methds-reagnts Subject: oligo mutagenesis Message-ID: Date: 13 Jun 91 22:46:48 GMT References: <9106131507.AA24715@uk.ac.aber.aberda> Distribution: bionet Organization: Carnegie Mellon, Pittsburgh, PA Lines: 11 In-Reply-To: <9106131507.AA24715@uk.ac.aber.aberda> Recently, two people in our lab have attempted HA-tagging using oligos; a third person is undertaking site-directed mutagenesis with an oligo. In at least two of the three cases, sequencing revealed mutations (insertions, rearrangements) in portions of the gene a considerable distance downstream of the site complementary to the oligo. Has anyone else had these difficulties? Does the source of the oligo make a difference? (i.e. a professional company vs. a university "part time" facility) Any comments are greatly appreciated! Elaine Weidenhammer