Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!wuarchive!uunet!stanford.edu!agate!bionet!jii.afrc.ac.uk!MURPHY
From: MURPHY@jii.afrc.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <9106260058.AA26184@genbank.bio.net>
Date: 25 Jun 91 19:18:00 GMT
Sender: daemon@genbank.bio.net
Distribution: bionet
Lines: 15

I heard recently of a method for detecting and quantifying RNA using short
complimentary oligos, labelled 5' with 32-P and with a nonsense 3' tail. After
hybridization the single-strand nonsense tail is digested with S1 nuclease and
the oligo gel-separated. Does anybody have a reference for this method, or
better still, a detailed protocol?
Thanks in advance.
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George Murphy                                  |Snailmail: Molecular Genetics,
JANET:murphy@uk.ac.afrc.jii                    |           Cambridge Laboratory,
INTERNET:murphy%jii.afrc.ac.uk@cunyvm.cuny.edu |           JI Centre for Plant
EARN/BITNET:murphy%jii.afrc.ac.uk@UKACRL       |           Science Research,
                                               |           Colney Lane,
Telephone: +44 603 52571                       |           Norwich NR4 7UJ,
Fax:       +44 603 502270                      |           U.K.
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