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From: ems@amdahl.UUCP (E. Michael Smith)
Newsgroups: net.bio
Subject: Re: HELP!
Message-ID: <1058@amdahl.UUCP>
Date: Mon, 28-Jan-85 15:08:18 EST
Article-I.D.: amdahl.1058
Posted: Mon Jan 28 15:08:18 1985
Date-Received: Wed, 30-Jan-85 05:38:20 EST
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Organization: Amdahl Corp, Sunnyvale CA
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> 
> Hey, do any of you pros have an opinion on how an amateur can sterilize his
> glassware to a level that is acceptable when he is working in a home-basement
> lab?

I'm not a pro, but that doesn't stop me from having an oppinion  ( in
the true tradition of the net...)

I think the oven is your best bet.  Given my 'experiments' with baked
chicken left in the oven overnight, it seems that the environment stays
reasonably sterile.  It should be that glassware would be sterile as soon
as the surface temperature reached 300 degrees F or so.  The biggest risk
is in contamination when the door is opened.

Also, have you thought of trying acetone as a sterilizer?  Evaporates
much better than ethanol.

Another trick I have used is to use isopropanol to dissinfect/sterilize
and then dry in the oven at 250 degrees.  Fast and efficient.

-- 

E. Michael Smith  ...!{hplabs,ihnp4,amd,nsc}!amdahl!ems

Comedo ergo sum

The opinions expressed by me are not representative of those of any
other person - natural, unnatural, or fictional - and only marginally
reflect my opinions as strained by the language.