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Path: utzoo!watmath!clyde!burl!ulysses!allegra!sjuvax!mccann
From: mccann@sjuvax.UUCP (mccann)
Newsgroups: net.bio
Subject: Re: HELP!
Message-ID: <782@sjuvax.UUCP>
Date: Fri, 1-Feb-85 14:11:55 EST
Article-I.D.: sjuvax.782
Posted: Fri Feb  1 14:11:55 1985
Date-Received: Sun, 3-Feb-85 11:05:53 EST
References: <1204@drusd.UUCP> <1058@amdahl.UUCP> <11808@gatech.UUCP>
Reply-To: mccann@sjuvax.UUCP (mccann)
Organization: St. Josephs University, Phila. PA.
Lines: 13



     I am not really sure that either the UV light bit or the use of an oven
at 350 will completely sterilize glassware. First, UV cannot significantly
penetrate glass, so this would be useless for sterilizing pipettes and such
(also, the UV varies greatly in effectiveness on different types of bacteria
and exposure of 'sterilized' glassware after irradiation would initiate photo-
reactivation of bacteria not yet dead.) The heating in an oven bit also is not 
totally effective, since even non-cysting bacteria can survive high temperatures
for some time. Your best bet is to go out and but yourself a new pressure cooker
because this saves time and will probably do a better job of sterilizing than
the other methods.
M McCann