Relay-Version: version B 2.10 5/3/83; site utzoo.UUCP Path: utzoo!mnetor!seismo!husc6!hao!boulder!eddy From: eddy@boulder.Colorado.EDU (Sean Eddy) Newsgroups: sci.bio Subject: Re: Life Classification ...further comments Message-ID: <1211@sigi.Colorado.EDU> Date: Sat, 30-May-87 15:40:24 EDT Article-I.D.: sigi.1211 Posted: Sat May 30 15:40:24 1987 Date-Received: Mon, 1-Jun-87 05:44:32 EDT References: <9543@duke.cs.duke.edu> <1125@ius2.cs.cmu.edu> <701@edge.UUCP> Sender: news@sigi.Colorado.EDU Reply-To: eddy@boulder.Colorado.EDU (Sean Eddy) Distribution: world Organization: University of Colorado, Boulder Lines: 30 In article <382@uhnix2.UUCP> bchso@uhnix2.UUCP (Dan Davison) writes: >In article <1991@husc6.UUCP> gallagher@husc4.UUCP (paul gallagher) writes: >>Prokaryotes exchange genetic information >>with plasmids... > >And via viruses. Bacillus sp. also pick up DNA directly from the environment, >ie from those around them that have died and lysed the cell membrane. Dan, is it true that only the Bacillus species can pick up and incorporate DNA from the environment? I was under the possibly naive impression that pretty much all the bacteria could do this trick, called transformation. The principle of bacterial transformation is the heart of genetic engineering in E. coli (admittedly, coli transformation is artificially enhanced in the laboratory), as well as the crucial point in the classical experiments of Avery, McCleod, and McCarty that indicated DNA is the genetic material (incidentally, their experiments used Diplococcus). I'll be interested to hear if my concept of transformation is indeed overly naive. - Sean Eddy - Dept. of Molecular, Cellular, Developmental Biology - Univ. of Colorado, Boulder; Boulder, CO 80309 - - "It's hard to argue with someone who knows what he's talking about." - - Craig Werner (MD/PhD '91) - "Everything you know is wrong." - - Firesign Theatre