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From: pell@boulder.Colorado.EDU (Anthony Pelletier)
Newsgroups: sci.bio
Subject: Re: Life Classification ...further comments
Message-ID: <1330@sigi.Colorado.EDU>
Date: Tue, 9-Jun-87 13:59:23 EDT
Article-I.D.: sigi.1330
Posted: Tue Jun  9 13:59:23 1987
Date-Received: Fri, 12-Jun-87 05:08:32 EDT
References: <9543@duke.cs.duke.edu> <1125@ius2.cs.cmu.edu> <701@edge.UUCP> <1302@sigi.Colorado.EDU> <1117@aecom.YU.EDU>
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Reply-To: pell@boulder.Colorado.EDU (Anthony Pelletier)
Organization: University of Colorado, Boulder
Lines: 16
Keywords: Hanahan Transformation

i wrote:
 It is well established that HB101 will not stand up to the Hanahan proceedure.
 It even says so in the "Joy of Cloning."
>
craig wrote:
>	I have gotten Hanahan transformation to work with HB101, as well
>as JM101.
>
This will teach me not to believe what I read.  Even the cloning manual can
be wrong.   It said not to try it on HB101 and I didn't.
The rest of what I said was from first-hand experience and therefore should
more closely approximate the truth.
Thanks for the info, and mea culpa.  
Also, thanks for the info regarding ph.  I never checked it before.

tony (Empire Truck Driving School grad--cum laude '92)