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From: bchso@uhnix2.UUCP
Newsgroups: sci.bio
Subject: Re: Life Classification ...further comments
Message-ID: <393@uhnix2.UUCP>
Date: Tue, 9-Jun-87 13:25:52 EDT
Article-I.D.: uhnix2.393
Posted: Tue Jun  9 13:25:52 1987
Date-Received: Sat, 13-Jun-87 08:48:51 EDT
References: <9543@duke.cs.duke.edu> <1125@ius2.cs.cmu.edu> <701@edge.UUCP> <1211@sigi.Colorado.EDU> <1105@aecom.YU.EDU>
Reply-To: bchso@uhnix2.UUCP (Dan Davison)
Organization: University of Houston
Lines: 18
Summary: transformation and "Liquid Gold"

In article <1105@aecom.YU.EDU> werner@aecom.YU.EDU (Craig Werner) writes:
> Douglas Hanahan,
>more recently, has described methods using Ca, Mn, Cobalt Hexamine
>Chloride, DMSO, and DTT, and other reagants that I can't recall right
>off, that boost transformation frequencies several orders of magnitude -
>to almost a percent or higher.

I was a grad student at SUNY Stony Brook and had occasion to hang out at
the Cold Spring Harbor Laboratory from time to time.  Doug Hanahan 
worked there and before he published the recipe the reagent mix mentioned
by proto-doctor Werner was commonly referred to as "liquid gold".


dr. dan davison/ Dept of Biochemical and Biophysical Sciences/ U. of Houston
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After July 1: T-10 MS K-710 Los Alamos National Laboratory, Los Alamos N.M.
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