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From: diaz@aecom.UUCP
Newsgroups: sci.bio
Subject: Divergent promoters in prokaryotes -- seeking advice
Message-ID: <1136@aecom.YU.EDU>
Date: Sun, 14-Jun-87 14:38:12 EDT
Article-I.D.: aecom.1136
Posted: Sun Jun 14 14:38:12 1987
Date-Received: Tue, 16-Jun-87 01:36:14 EDT
Organization: Graduate School of Hard Knocks
Lines: 22
Keywords: Transcription initiation; co-regulation; RNA polymerase


I am currently involved in the study of an E. coli gene whose
promoter region is very close to, and may in fact overlap with the
promoter of a second gene. The two genes are transcribed in opposite
directions. We are interested in ascertaining whether transcription of
one gene has an effect on transcription of the other. One idea is to
measure the level of message for one of the genes while the second is
repressed, and again while it is derepressed.

I was wondering if anyone knows of any other examples of well
characterized prokaryotic divergent promoters, so that I can pick up
some clues from the work of others. Any ideas Craig? I've been told that
there is an example of promoter divergence in the Arg biosynthesis loci,
and recently at the ASBC meeting in Philly, I saw a poster on a tRNA
gene whose promoter was close to a divergently transcribed tRNA
synthetase gene.


-- 
5'gtacggagc dn/dx = Dan Diaz    (philabs!aecom!diaz)
            Department of Molecular Biology & Snake Oil Dynamics 
            Albert Slimestein College of Medicine ctataacagcta 3'