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From: diaz@aecom.UUCP
Newsgroups: sci.bio
Subject: Cloning/Expression vector copy numbers
Message-ID: <1137@aecom.YU.EDU>
Date: Sun, 14-Jun-87 14:45:43 EDT
Article-I.D.: aecom.1137
Posted: Sun Jun 14 14:45:43 1987
Date-Received: Tue, 16-Jun-87 01:36:25 EDT
Organization: Graduate School of Hard Knocks
Lines: 16
Keywords: Plasmids; Gene amplification; Protein overexpression


A post-doctoral fellow here at Einstein informed me recently that the
Gemini series of plasmids marketed by Promega Biotech (pGEM1, 2, 3, 4,
and blue) have copy numbers on the order of 400 per cell. This is one of
the highest copy numbers I have heard of. We may try to clone an E. coli
gene into one of these plasmids in order to see whether we can get
increased levels of an enzyme we're studying just on the basis of copy
number. 

Does anyone know of any plasmids with even higher copy numbers? If so,
where are they available from, and what is their selection?

-- 
5'gtacggagc dn/dx = Dan Diaz    (philabs!aecom!diaz)
            Department of Molecular Biology & Snake Oil Dynamics 
            Albert Slimestein College of Medicine ctataacagcta 3'