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From: werner@aecom.UUCP
Newsgroups: sci.bio
Subject: Re: Divergent promoters in prokaryotes -- seeking advice
Message-ID: <1140@aecom.YU.EDU>
Date: Mon, 15-Jun-87 00:23:46 EDT
Article-I.D.: aecom.1140
Posted: Mon Jun 15 00:23:46 1987
Date-Received: Thu, 18-Jun-87 04:54:14 EDT
Organization: Albert Einstein Coll. of Med., NY
Lines: 32
Keywords: Transcription initiation; co-regulation; RNA polymerase

(Dizzy Dan Diaz) writes:
> 
> I am currently involved in the study of an E. coli gene whose
> promoter region is very close to, and may in fact overlap with the
> promoter of a second gene. The two genes are transcribed in opposite
> directions.
> 
> I was wondering if anyone knows of any other examples of well
> characterized prokaryotic divergent promoters, so that I can pick up
> some clues from the work of others. Any ideas Craig? 
	                                         ^- I can't resist
a direct invitation to post!


	The best study of divergent promoters in E. coli, down to
the molecular details is the arabinose operon, where araC, the
Positive regulator/Repressor goes one way, and araBAD goes the other.

	And let's not forget P(R) and P(RM), for Promoter(Right)
and Promoter(Repressor Maintenance) of the bacteriophage lambda,
where cI and cro differential binding on OR1, OR2, and OR3,
are responsible for the lytic/lysogenic switch.  This has the
advantage that Mark Ptashne just wrote a book about it, and
it's out in paperback.  It's well written, and everybody should
buy it because Ptashne still has some payments left on his
Stradavarius.

-- 
			      Craig Werner (MD/PhD '91)
				!philabs!aecom!werner
              (1935-14E Eastchester Rd., Bronx NY 10461, 212-931-2517)
               "Why is it that half the calories is twice the price?"