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From: bchso@uhnix2.UUCP (Dan Davison)
Newsgroups: sci.bio
Subject: More on natural transformation
Message-ID: <401@uhnix2.UUCP>
Date: Tue, 16-Jun-87 23:19:04 EDT
Article-I.D.: uhnix2.401
Posted: Tue Jun 16 23:19:04 1987
Date-Received: Thu, 25-Jun-87 06:29:32 EDT
Organization: University of Houston
Lines: 22
Keywords: CaCl2, philosophy

[List of organisms that can be transformed]
>>Gee, Sean, I consider this a handful.  But this is a difference in
>>philosophy, since the mammalian cell culture transformation you cite
>>would never be the same transformation as bacterial, *to me*.

>Why not? I don't mean 'growth transformation' or 'neoplastic
>transformation', 

Understood.

>I'm just referring to the uptake and integration
>of exogenous DNA fragments.

Because the conditions under which mammalian cells uptake DNA, and
under which E. coli do the same are not very likely to occur in nature.
The Gram-positive style of transformation can occur in soil, whereas
the calcium chloride bath that E. coli requires is less likely to be 
be found in its natural environment.

dan davison/ bchs6@uhupvm1.bitnet/ davison@bionet-20.arpa / davison@bnl.arpa
              uucp: ...rice!academ!uhnix1!uhnix2!bchso
         "Of all the things I've lost I miss my mind the most"