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From: bchso@uhnix2.UUCP (Dan Davison)
Newsgroups: sci.bio
Subject: Re: Cloning/Expression vector copy numbers
Message-ID: <404@uhnix2.UUCP>
Date: Fri, 19-Jun-87 23:31:57 EDT
Article-I.D.: uhnix2.404
Posted: Fri Jun 19 23:31:57 1987
Date-Received: Thu, 25-Jun-87 06:34:41 EDT
References: <1137@aecom.YU.EDU>
Reply-To: bchso@uhnix2.UUCP (Dan Davison)
Organization: University of Houston
Lines: 11
Keywords: Plasmids; Gene amplification; Protein overexpression

In article <1137@aecom.YU.EDU> diaz@aecom.YU.EDU (Dizzy Dan) writes:
>Does anyone know of any plasmids with even higher copy numbers? If so,
>where are they available from, and what is their selection?

Have you considered and rejected chloramphenicol amplification?  I've heard
it can get 1000s (!) per cell.  The plasmid must be descended from ColE1,
though.

dr. dan davison|Los Alamos National Laboratory|Theoretical Biology|T-10,
MS K-710, Los Alamos, NM 87545...rice!academ!uhnix1!uhnix2!bchso
"Of all the things I've lost I miss my mind the most"