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From: diaz@aecom.YU.EDU (Dizzy Dan Diaz)
Newsgroups: sci.bio
Subject: Re: Cloning/Expression vector copy numbers
Message-ID: <1160@aecom.YU.EDU>
Date: Thu, 25-Jun-87 21:56:38 EDT
Article-I.D.: aecom.1160
Posted: Thu Jun 25 21:56:38 1987
Date-Received: Sun, 28-Jun-87 00:56:33 EDT
References: <1137@aecom.YU.EDU> <404@uhnix2.UUCP>
Organization: Graduate School of Hard Knocks
Lines: 20
Summary: Chloramphenicol not appropriate for expression

In article <404@uhnix2.UUCP>, bchso@uhnix2.UUCP (Dan Davison) writes:

> In article <1137@aecom.YU.EDU> diaz@aecom.YU.EDU (Dizzy Dan) writes:
> >Does anyone know of any plasmids with even higher copy numbers? If so,
> >where are they available from, and what is their selection?
> 
> Have you considered and rejected chloramphenicol amplification?  I've heard
> it can get 1000s (!) per cell.  The plasmid must be descended from ColE1,
> though.

If I were simply interested in making a plasmid preparation
chloramphenicol would serve my purposes. What I'm interested in,
however, is observing whether I can get increased cloned enzyme gene
expression levels based on copy number. Chloramphenicol would increase
the copy number, but it would also interfere with protein synthesis.

-- 
   ....     dn/dx = Dan Diaz (philabs!aecom!diaz or diaz@aecom.yu.edu)
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