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From: diaz@aecom.YU.EDU (Daniel Diaz)
Newsgroups: sci.bio
Subject: Isolation of single-stranded DNA
Message-ID: <1412@aecom.YU.EDU>
Date: Thu, 5-Nov-87 00:07:01 EST
Article-I.D.: aecom.1412
Posted: Thu Nov  5 00:07:01 1987
Date-Received: Sun, 8-Nov-87 02:57:48 EST
Distribution: na
Organization: Graduate School of Hard Knocks
Lines: 13
Keywords: S1 analysis, electrophoresis

In an attempt to avoid the complications of probe renaturation during S1
nuclease mapping of transcription start sites, I wish to easily isolate
single-stranded DNA from labeled duplex.  The new Guide to Molecular
Cloning Techniques put out by our friends at Methods in Enzymology has
not been helpful in this regard.  Although helpful, I've found the book
in need of excision repair.

Electrophoresis in polyacrylamide, 7M urea has been used by some, but I
don't know how well it works.  Recloning into m13 would be easiest, but
time-consuming.  Please send any suggestions via email.
-- 
      dn/dx      Dept Molecular Biology   diaz@aecom.yu.edu
     Dan Diaz           AECOM           "I've met Craig Werner"