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From: diaz@aecom.YU.EDU (Dizzy Dan Diaz)
Newsgroups: sci.bio
Subject: Re: Isolation of single-stranded DNA
Message-ID: <1427@aecom.YU.EDU>
Date: Thu, 12-Nov-87 17:24:44 EST
Article-I.D.: aecom.1427
Posted: Thu Nov 12 17:24:44 1987
Date-Received: Sun, 15-Nov-87 18:48:53 EST
References: <1412@aecom.YU.EDU> <1370@cup.portal.com>
Organization: Graduate School of Hard Knocks
Lines: 15

In article <1370@cup.portal.com>, Mitchell_K_Hobish@cup.portal.com writes:
> Have you considered cesium chloride (or the equivalent) ultracentrifu-
> gation?  It used to work well for me when isolating polyoma or SV-40
> DNA in supercoiled, open circle, nicked, etc. forms...

CsCl does not quite suit the purpose I had in mind.  I was interested in
separating strands of 5' labelled DNA frgaments < 1kb long.  CsCl by
itself would be unsuitable for large-scale preparations anyway since
CsCl it nondenaturing.  Alkaline gradients would be best for that
purpose.  Thanks for the suggestion anyway.
 

-- 
      dn/dx      Dept Molecular Biology   diaz@aecom.yu.edu
     Dan Diaz      Albert Finkelstein College of Quackery