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From: werner@aecom.YU.EDU (Craig Werner)
Newsgroups: sci.bio
Subject: Re: Using the slow form of Bal 31 Nuclease
Message-ID: <1446@aecom.YU.EDU>
Date: Thu, 12-Nov-87 23:30:43 EST
Article-I.D.: aecom.1446
Posted: Thu Nov 12 23:30:43 1987
Date-Received: Sun, 15-Nov-87 18:49:54 EST
References: <1445@aecom.YU.EDU>
Organization: Albert Einstein Coll. of Med., NY
Lines: 16
Keywords: Deletion analysis, controlled digestions

In article <1445@aecom.YU.EDU>, diaz@aecom.YU.EDU (Dizzy Dan Diaz) writes:
> Bal 31 nuclease as prepared is much too difficult to control for my
> purposes.  

	In certain more relaxed labs on the western spiral arm of the
Galaxy, the double stranded chewing of Bal31 has been completely
superceded by exoIII followed by single-stranded exonuclease.  The
exoIII chews one strand 3'->5' under reasonable control, and the 
resulting frayed end is subsequently blunt ended.  Quite clever,
actually.

-- 
	        Craig Werner   (future MD/PhD, 3 years down, 4 to go)
	     werner@aecom.YU.EDU -- Albert Einstein College of Medicine
              (1935-14E Eastchester Rd., Bronx NY 10461, 212-931-2517)
      "... Has Determined That Cigarette Smoking Is Dangerous To Your Health"