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From: diaz@aecom.YU.EDU (Dizzy Dan Diaz)
Newsgroups: sci.bio
Subject: Re: Using the slow form of Bal 31 Nuclease
Message-ID: <1451@aecom.YU.EDU>
Date: Fri, 13-Nov-87 14:15:55 EST
Article-I.D.: aecom.1451
Posted: Fri Nov 13 14:15:55 1987
Date-Received: Sun, 15-Nov-87 18:52:10 EST
References: <1445@aecom.YU.EDU> <1446@aecom.YU.EDU>
Organization: Graduate School of Hard Knocks
Lines: 16
Keywords: Deletion analysis, controlled digestions
Summary: Just the facts, sir

In article <1446@aecom.YU.EDU>, werner@aecom.YU.EDU (Craig Werner) writes:
> 	In certain more relaxed labs on the western spiral arm of the
> Galaxy, the double stranded chewing of Bal31 has been completely
> superceded by exoIII followed by single-stranded exonuclease.  The
> exoIII chews one strand 3'->5' under reasonable control, and the 
> resulting frayed end is subsequently blunt ended.  Quite clever,
> actually.

Fine Werner, that's just great, but how's about some NUMBERS, eh?  Under
reasonable control could mean 400 nt min^-1 or 2 nt min ^-1.  If you're
going to be of help, you must be complete about it.  Besides, I don(t
want to look it up myself.

-- 
      dn/dx      Dept Molecular Biology   diaz@aecom.yu.edu
     Dan Diaz      Albert Grubelstein College of Osteopathy