Path: utzoo!utgpu!water!watmath!uunet!ig!daemon From: SOFER@BIONET-20.ARPA Newsgroups: bionet.general Subject: Course announcement Message-ID: <4989@ig.ig.com> Date: 4 Feb 88 18:48:25 GMT Sender: daemon@presto.ig.com Lines: 41 From: William H. Sofer Rapid Cloning of Genes Encoding Sequence-specific DNA Binding Proteins A Workshop in Molecular Genetics and Biotechnology Presented by Drs. Steven L. McKnight, Karen LaMarco and Charles Vinson April 21-22, 1988 At the Waksman Institute, Rutgers University Piscataway, NJ 08854-0759 201-932-4258 This course is intended for those actively engaged in the field of eukaryotic gene regulation, and who are interested in a new (unpublished) technique for the cloning of genes that code for proteins that bind to DNA. One of the goals of those studying gene regulation is to identify and purify proteins that bind to DNA in a sequence- specific manner. These trans-regulatory proteins, by binding to cis-regulatory DNA sequences located within promoter and enhancer elements, play an important role in the regulation of gene expression. Dr. McKnight and his colleagues have developed a technique that has allowed them to screen directly for lambda gt-11 clones that encode proteins that bind to specific DNA sequences. Their procedure, which involves several simple modifications of standard lambda gt-11 screening, offers a potentially rapid method for the isolation of genes that encode rare DNA binding proteins. The two day course will cover a start-to-finish screening procedure, offering hands-on experience with DNA probes, filter lifts and treatment of culture plates. At the end of the course each participant will be provided with a sample of the lambda gt-11 bacteriophage that encodes the protein that serves as a positive control. -------