Path: utzoo!utgpu!water!watmath!uunet!ig!daemon
From: EBEALE@BIONET-20.ARPA
Newsgroups: bionet.general
Subject: Promoter without cell specificity
Message-ID: <5685@ig.ig.com>
Date: 30 Mar 88 17:35:45 GMT
Sender: daemon@presto.ig.com
Lines: 14

From: Elmus Beale <EBEALE@BIONET-20.ARPA>

   We are using transient expression assays to investigate the mechanisms
that control cell-specific gene expression.  We need a control vector
that is efficiently expressed in all or most cell types.  We have used
enhancer/promoters from SV40, RSV, metallothionein, but these show
tissue-preferences.  Perhaps there is not a perfect control but I
would think that the promoters from housekeeping genes such as tubulins,
beta-actin, HPRT, or one of the histones might work.
    If anyone has suggestions, and (better yet) genomic clones of
such genes please contact me.
    Thanks,
    Elmus Beale
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