Path: utzoo!utgpu!water!watmath!uunet!ig!daemon
From: CABELL.ANDREWS@BIONET-20.ARPA
Newsgroups: bionet.molbio.seqnet
Subject: To Douglas Kell/University College,Wales
Message-ID: <5741@ig.ig.com>
Date: 4 Apr 88 14:43:14 GMT
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From: Paul Andrews <CABELL.ANDREWS@BIONET-20.ARPA>

Douglas Kell:re-FAD synthesis with C. kluyveri

             Sorry I have been so long in replying, but I have been out of town
for a while and haven't had a chance to check my mail.  To remind you of my 
problem.....I am interested in synthesizing 2-C14-FAD from 2-C14-ribolflavin.  
One published procedure is to incubate Clostridium kluyveri with C14-riboflavinand then isolate C14-FAD from the cell pellet.  I have had a few other 
suggestions from other groups and apparently the approach used now is to 
produce an "FAD synthetase" complex from Brevibacterium ammoniagenes.  I am 
going to try this approach first before attempting to grow more bacteria.  
anyway, if I have anymore trouble I may be in touch.  Thankyou for your 
responce.
                        Yours truly (..et merci from a displaced Canadian...)
                        Paul Andrews
                        College of Pharmacy/Medicinal Chemistry
                        University of Texas at Austin
                        Austin, Texas
                        78712
                        (EMAIL CABELL.ANDREWS)
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