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Date: 5 Apr 88 11:51:47 GMT
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From:    Paul Andrews <CABELL.ANDREWS@arpa.bionet-20>  4-APR-1988 15:50
To:    SEQNET
Subj:    To Douglas Kell/University College,Wales


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From: Paul Andrews <CABELL.ANDREWS@arpa.bionet-20>
Subject: To Douglas Kell/University College,Wales
To: seqnet@arpa.bionet-20
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Douglas Kell:re-FAD synthesis with C. kluyveri

             Sorry I have been so long in replying, but I have been out of town
for a while and haven't had a chance to check my mail.  To remind you of my
problem.....I am interested in synthesizing 2-C14-FAD from 2-C14-ribolflavin.
One published procedure is to incubate Clostridium kluyveri with C14-riboflavina
suggestions from other groups and apparently the approach used now is to
produce an "FAD synthetase" complex from Brevibacterium ammoniagenes.  I am
going to try this approach first before attempting to grow more bacteria.
anyway, if I have anymore trouble I may be in touch.  Thankyou for your
responce.
                        Yours truly (..et merci from a displaced Canadian...)
                        Paul Andrews
                        College of Pharmacy/Medicinal Chemistry
                        University of Texas at Austin
                        Austin, Texas
                        78712
                        (EMAIL CABELL.ANDREWS)
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