Path: utzoo!mnetor!uunet!lll-winken!lll-lcc!ames!umd5!mimsy!deba From: deba@mimsy.UUCP (Deba Patnaik) Newsgroups: sci.bio Subject: Forwarded Msg Message-ID: <11123@mimsy.UUCP> Date: 19 Apr 88 23:03:02 GMT Organization: U of Maryland, Dept. of Computer Science, Coll. Pk., MD 20742 Lines: 12 From: Michael Clarke <37040_442@uwovax.UWO.CDN> Subject: Help "We have been experiencing problems with amino terminal blockage of proteins purified by gel electrophoresis prior to sequencing. I recall seeing a method for the reversible blocking of primary amino groups with FMOC as a protective strategy before electrophoresis. Can anyone help me by supplying either the method or a reference to such a method? I would be very grateful. Please send info to: Michael Clarke@uwovax.uwo.cdn or Michael Clarke@uwovax.bitnet"