Path: utzoo!mnetor!uunet!husc6!mailrus!ames!oliveb!pyramid!prls!philabs!aecom!diaz
From: diaz@aecom.YU.EDU (Dizzy Dan)
Newsgroups: sci.bio
Subject: Antisense-RNA-derived mutants
Message-ID: <1784@aecom.YU.EDU>
Date: 5 May 88 02:57:38 GMT
Organization: Graduate School of Hard Knocks
Lines: 10

I am interested in generating mutant strains of E. coli in two genes
I've been working with.  I don't want to mutagenize and hunt for mutants
(yuck, what a mess!).  The method I use should be simple, effective, and
specific to the genes in question.  What if I put an antisense code
for the gene(s) on a plasmid and transform.  The antisense message would
then prevent translation of the sense message, right?  I'd appreciate some
advice, although, as always, Dan Davison may not respond.
-- 
      dn/dx      Dept Molecular Biology   diaz@aecom.yu.edu
     Dan Diaz      Albert Grubelstein College of Osteopathy