Path: utzoo!utgpu!water!watmath!uunet!ig!daemon From: elliston@biovax.rutgers.edu Newsgroups: bionet.molbio.methds-reagnts Subject: sequencing methods... Message-ID: <6285@ig.ig.com> Date: 17 May 88 01:11:28 GMT Sender: daemon@presto.ig.com Lines: 25 From: To All DNA Sequencers: I am looking for everyones little "favorite" tips for sequencing. I am putting together a little manual on sequencing and thought that it would be nice to include all the "tricks of the trade" that people on the Nets thought were of use. One particular thing I am looking for, is a method for using formamide gels (rather than Urea). I have heard that these are good for resolving compressions, but cant find a recipe or electrophoresis conditions anywhere. If I get some good responses and/or there is interest, I will summarize the results to the net. Thanx. Keith ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Keith O. Elliston | Waksman Institute Bitnet: ELLISTON@BIOVAX | P.O. Box 759 Arpanet: ELLISTON@biovax.rutgers.edu | Rutgers, The State U. of NJ AT&T: (201)932-3801 | Piscataway, NJ 08855-0759 USA ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ------