Path: utzoo!attcan!uunet!husc6!mailrus!ames!ncar!boulder!eddy
From: eddy@boulder.Colorado.EDU (Sean Eddy)
Newsgroups: sci.bio
Subject: Re: Bizarre DNA similarity
Message-ID: <6639@sigi.Colorado.EDU>
Date: 14 Jun 88 13:47:43 GMT
References: <1843@aecom.YU.EDU>
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In article <1843@aecom.YU.EDU> werner@aecom.YU.EDU (Robert N. Berlinger) writes:
>	The result was astounding:  the reverse complement of Human
>ADH Class I Beta, WITH those two mistakes is in 130 base pairs 100%
>similar to part of the intergenic region of pBR322.  It absolutely
>blew me away.  If someone had told me that two pieces of essentially
>unrelated DNA could be identical over 130 bases I would have said that
>they were crazy, but I saw it with my own eyes. Amazing.

In fact, I'm so amazed I'd be tempted to find out how ADH class I beta
was sequenced. Like, for instance, was it in (or subcloned from)
a pBR vector?? 130 bp of 100% sequence similarity suggests a major
muck-up...

- Sean Eddy
- Molecular/Cellular/Developmental Biology; U. of Colorado at Boulder
- eddy@boulder.colorado.EDU		!{hao,nbires}!boulder!eddy	
-
- "That such pygmies should cast such giant shadows only shows how
-  late in the day it has become."
-                       - biochemist Erwin Chargaff, of Watson & Crick