Path: utzoo!attcan!uunet!munnari!murdu!sam From: sam@murdu.OZ (Sam Ganesan) Newsgroups: sci.bio Subject: Free radical footprinting Keywords: Fe-EDTA, footprinting Message-ID: <1396@murdu.OZ> Date: 19 Jun 88 09:40:57 GMT Organization: Comp Sci, Melbourne Uni, Australia Lines: 19 Does anybody out there have experience on free radical cutting of DNA for foot printing studies. I know people have used DNAse footprinting for quite a while but from what I gather hydroxyl free radicals generated using Fe-EDTA and hydrogen peroxide have less sequence specific cutting (if any). Any help on technical details and methodology gratefully accepted. Dan Davison, You out there? Whats the word on this? - Sam Ganesan ############################################################################### ACSnet: sam@murdu.oz JANET: sam%murdu.mu.oz@uk.ac.ukc ARPA: sam%murdu.mu.oz.au@uunet.uu.net UUCP: {uunet,hplabs,mcvax,ukc,nttlab}!munnari!murdu.oz.au!sam Snail: Sam Ganesan,Dept of Microbiology,Melbourne Uni,Parkville,Victoria 3052 Australia. ###############################################################################