Path: utzoo!utgpu!water!watmath!uunet!ig!relay.cs.net!wallace%fmi.ch
From: wallace%fmi.ch@RELAY.CS.NET (Andrew Wallace)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Uniform gels for reading long sequences
Message-ID: <196*wallace@FMI.CH>
Date: 29 Jul 88 10:09:00 GMT
References: <12412284971.17.EROSENTHAL@BIONET-20.ARPA>
Sender: daemon@presto.ig.com
Lines: 15

Eric,
	Regarding your query about the sticking of the X-Ray film to gels,
how do you fix your gels before drying ? I get excellent results by using
12%(v/v) methanol/10% (v/v) acetic acid /water, which I apply to the gel
by use of a wash-bottle (not leaving the gel swimming in a bath of the 
stuff - this doesn't work and you risk breaking the gel). The gel and plate
are supported above a tray or sink to catch the run-off from the gel, and
I just rinse the gel with the fixing solution every 5 minutes for a half-
hour. The gel is supported so that it does not sit in contact with old 
solution. I find that this method gives the best results to remove urea
from the gel prior to drying, otherwise the urea crystallises on the
surface of the gel, causing the sticking and the little black spots.
Best wishes,
	Andrew Wallace, FMI, Basel, Switzerland.
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