Path: utzoo!utgpu!water!watmath!uunet!ig!cunyvm.cuny.edu!CHULYANG%KRSNUCC1.BITNET From: CHULYANG%KRSNUCC1.BITNET@CUNYVM.CUNY.EDU (jaewon soh) Newsgroups: bionet.general Subject: hydrogenase Message-ID: <8808221341.AA25621@presto.ig.com> Date: 22 Aug 88 13:41:15 GMT Sender: daemon@presto.ig.com Lines: 29 Dir BIONET members, I am a Korean biochemistry student at SEOUL National University. I am very happy to have people to talk about my problem. I am working on the hydrogenase system in E.coli and Desulfovibrio. Hydrogenase catalyzes the simple reaction : "2H + 2e- <====> H2" And electron carrier is involved (eg. cytochrome or NADH). I isolated the hydrogenase gene and cloned and I'm on sequencing now. Sequencing is almost finished. Now I am eager to understand more of the hydrogenase system. But environment puts limit on me. All tools that I have are only two PC softwares (PC-Sequence & Mount's). I hope to search homology in DNA and amino acid data bank and to predict its secondary structure. I know the new program called FASTA-MAIL on BIONET. But I cannot login BIONET because I am on BITNET. I can only receive and send bboard messages. Will you please help me? If you are inclined to help me, tell me please. Then I'll send you my sequence data by E-mail. I welcome anyone's advise and help and I'll be very happy. Thanks in advance. Sincerely Yours, Jaewon Soh * E-mail address BITNET; CHULYANG@KRSUNCC1 via ARPANET; CHULYANG@KRSUNCC1.BITNET or CHULYANG%KRSUNCC1.BITNET@BERKELEY.ARPA