Path: utzoo!utgpu!watmath!uunet!bionet!vax.leicester.ac.uk!RAY
From: RAY@vax.leicester.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <8812182026.AA10562@net.bio.net>
Date: 16 Dec 88 09:42:19 GMT
Sender: daemon@NET.BIO.NET
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We are having terrible problems with insoluble cDNA. Every time we make double
stranded cDNA more than 50% of it sticks to the tubes as judged by the number
of counts recovered. We have tried siliconising the tubes but it did not help.
We have tried incubating the tubes in water baths at various temperatures but
makes no difference. I would be very grateful for any comments, cures etc....

Thanks,

Raymond Dalgleish,
Department of Genetics,
University of Leicester,
University Road,
Leicester LE1 7RH
U.K.
tel: +44(0533)523425