Path: utzoo!utgpu!watmath!uunet!labrea!rutgers!orstcs!bionette.ucs.orst.edu!chend
From: chend@bionette.ucs.orst.edu (Don Chen - Microbiology)
Newsgroups: bionet.molbio.methds-reagnts
Subject: coating lps on elisa plates
Message-ID: <8211@orstcs.CS.ORST.EDU>
Date: 12 Jan 89 06:08:38 GMT
Sender: usenet@orstcs.CS.ORST.EDU
Reply-To: chend@bionette.ucs.orst.edu (Don Chen - Microbiology)
Organization: Oregon State University - CMBL
Lines: 25


Hi:

My question involves the best method of coating LPS on ELISA
plates. I have extracted LPS from Vibrio (method of Darveau 
and Hancock). I believe the way to go is to put various
dilutions of the antigen in a solution of coating buffer
and incubate at 37 degrees C. I would follow with 3% BSA in
Tween-TBS, various sera to be tested, mouse monoclonal to
the Igs, goat anti-mouse-peroxidase, then developer.
Our sera are from various salmonids.

Is this correct? Have I most efficiently coated the LPS?
(We use Costar EIA/RIA plates). Are there references I should
read? What problems should I be aware of?

Thanks for any help you can give me.

Don Chen
Dept of Microbiology
Oregon State University
Corvallis, OR 97331
(503) 754-3189

email: chend@bionette.ucs.orst.edu