Path: utzoo!utgpu!watmath!uunet!bionet!bionet-20.bio.net!GKOHLHAW
From: GKOHLHAW@BIONET-20.BIO.NET (Gunter B. Kohlhaw)
Newsgroups: bionet.molbio.yeast
Subject: About two plasmids in one cell
Message-ID: <12461213323.21.GKOHLHAW@BIONET-20.BIO.NET>
Date: 9 Jan 89 18:09:12 GMT
Sender: daemon@NET.BIO.NET
Lines: 13

Dear fellow:

I am using two plasmids in one yeast cell to study the function of one gene
product.  

What could happen in a yeast cell that contains two plasmids?  One of the plasmids contains 2 micron sequence and a TRP1 marker;  the other one has a centromere sequence and a URA3 marker.  Both plasmids have an ampicilin resistant gene.  They are maintained in the cell by mutations of ura3 and trp1 in the genome.

I am glad that some body could give me some information about this issue.

			sciencerely,
				
			Kemin Zhou
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