Path: utzoo!utgpu!watmath!iuvax!bionet!bionet-20.bio.net!CABELL.ANDREWS
From: CABELL.ANDREWS@BIONET-20.BIO.NET (Paul Andrews)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Sulfo-EGS linker
Message-ID: <12466942681.13.CABELL.ANDREWS@BIONET-20.BIO.NET>
Date: 31 Jan 89 14:41:30 GMT
Sender: daemon@NET.BIO.NET
Lines: 37

Use of Sulfo-EGS as a Linker:

      I am interested in using a homobifunctional NHS ester: Sulfo-EGS
[4-ethlyene glycoBIS-(sulfosuccininmidyl succinate)], to link a peptide (20 Aa 
long) to albumin.  Because the 20-mer by itself is too water soluble to be 
precitated by TCA, linking the 20-mer to albumin should result in a complex 
that is easily precipitated.  I chose Sulfo-EGS (a new reagent from Pierce) 
because it is water soluble, reacts on with primary amino groups, and is 
easily cleaved with hydroxylamine.  The whole purpose of this experiment is to 
do some enzyme mediated coupling of a co-factor to a cysteine residue on the 
20-mer.  My problem is that I don't have a lot of this 20-mer (it is VERY 
expensive) and don't want to waste a lot of it determining optimum conditions 
for linking of the 20-mer to albumin.  Sulfo-EGS is such a new reagent that 
there are very few reports of its use in the literature.................
..............I was hoping that if someone has used this reagent as linker, or 
has had experience in making peptide-protein links, they could send me a 
protocol,.....or at least comment on whether this idea would work, or if 
there are alternate methods of linking the 20-mer to a protein.

                          Thanks,


                          Paul Andrews


============================================================================= 


Paul Andrews                         #  EMAIL:CABELL.ANDREWS@BIONET-20.BIO.NET 
                                     #       :CABELL.ANDREWS@[128.92.192.5]
University of Texas at Austin        #  Phone: (512) 471-3659, 471-1731
College of Pharmacy                  #
Division of Medicinal Chemistry      #
Austin, Texas, USA
78712

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