Path: utzoo!utgpu!watmath!iuvax!bionet!leicester.ac.uk!RAY
From: RAY@leicester.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Message-ID: <8904111036.AA21637@net.bio.net>
Date: 11 Apr 89 10:25:53 GMT
Sender: daemon@NET.BIO.NET
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Can anybody help us with idesas concerning the cloning of DNA fragments
produced by PCR amplification? We are really struggling to produce clones
in plasmid vectors and suspect that the problem may slight exonuclease
activity in the Taq polymerase. Has anybody else experienced this problem -
three independent groups here in Leicester are having the same problem.
We are going to try using Perkin-Elemer/Cetus AmpliTaq which should be free
of thermostable exonucleases but we are concernred that we may be barking
up the wrong tree.
Any help gratefully received.
Raymond Dalgleish,
Department of Genetics,
University of Leicester,
Leicester LE1 7RH, U.K.