Path: utzoo!utgpu!watmath!iuvax!bionet!bionet-20.bio.net!JROSENBAUM.CURRY
From: JROSENBAUM.CURRY@BIONET-20.BIO.NET (Alice Curry)
Newsgroups: bionet.molbio.methds-reagnts
Subject: ExoIII deletions
Message-ID: <12485361821.14.JROSENBAUM.CURRY@BIONET-20.BIO.NET>
Date: 11 Apr 89 21:00:52 GMT
Sender: daemon@NET.BIO.NET
Lines: 11

Is anyone out there familiar with the exoIII/S1 nuclease protocol of
Henikoff (Methods in Enzymology 155:156)?  I am currently having problems
with this protocol as implemented in Promega's Eraseabase kit.  Ironically,
we have used this kit for two previous projects with great success, but the
method, even with fresh components, isn't working anymore. The problems
semm to be occuring after the exo deletions themselves.  
  I'd appreciate hearing form anyone who has worked out problems with this
system, (with or without Promega's kit), or from anyone who has had problems
with the kit, particularly in recent months.
--Alice Curry
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