Path: utzoo!attcan!uunet!bionet!agate!eos!ames!haven!uvaarpa!galen!krebs.acc.Virginia.EDU!lah1l
From: lah1l@krebs.acc.Virginia.EDU (Lawrence A. Hite)
Newsgroups: bionet.molbio.methds-reagnts
Subject: cDNA Synthesis kits
Message-ID: <440@galen.acc.virginia.edu>
Date: 13 Apr 89 19:25:31 GMT
Sender: acc@galen.acc.virginia.edu
Reply-To: lah1l@krebs.acc.Virginia.EDU (Lawrence A. Hite)
Organization: University of Virginia, Dept. of Microbiology
Lines: 18

I am interested in finding out if anyone has any
experience with random priming (vs. oligo dT priming)
in the construction of a cDNA library.  Promega has
a new kit out based on random priming which they claim
will make cDNA from non-polyA RNA such as histone RNA
(this would not be important in my case as I will be
using oligo dT-selected RNA) and they also claim that 
the average length of clones is larger.  Has anyone
done side-by side tests of this, and if so what are
the advantages/disadvantages with respect to average
length, total yield and efficiency of ligagtion of 
linkers and vector?


L A Hite
UVa Medical School, Department of Microbiology
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