Path: utzoo!attcan!uunet!bionet!bionet-20.bio.net!JROSENBAUM.CURRY From: JROSENBAUM.CURRY@BIONET-20.BIO.NET (Alice Curry) Newsgroups: bionet.molbio.methds-reagnts Subject: non-radioactive labelling Message-ID: <12498667858.14.JROSENBAUM.CURRY@BIONET-20.BIO.NET> Date: 1 Jun 89 15:13:06 GMT Sender: daemon@NET.BIO.NET Lines: 8 Our lab is thinking about using non-radioactive labelling methods, such as biotinylated probes, for genomic southerns, northerns, and library screening. But we haven't found a lot of people with experience using them. I'd appreciate some advice on the subject-- what methods and kits have proved useful, which were not useful, how the costs compared to isotope labelling, was it worth making the switch? --Alice Curry -------