Path: utzoo!utgpu!watmath!uunet!bionet!bionet-20.bio.net!HUBERMAN
From: HUBERMAN@BIONET-20.BIO.NET (JOEL A. HUBERMAN)
Newsgroups: bionet.molbio.methds-reagnts
Subject: re: non-radioactive labelling
Message-ID: <12499189758.16.HUBERMAN@BIONET-20.BIO.NET>
Date: 3 Jun 89 14:59:59 GMT
Sender: daemon@NET.BIO.NET
Lines: 15

Alice,

     We have a limited amount of experience with the "Genius" kit from
Boehringer-Mannheim.  On good days it performs as advertised (0.1 pg
detected).  On bad days, it's sensitivity may be as much as 10-fold lower. 
Nylon membranes can be reprobed.  For many purposes, it's as good or better
than 32-P labelling, and, of course, it's a lot safer.  I haven't done a
detailed cost accounting, but, since the probes are stable and can be used
repeatedly, it's probably less expensive than 32-P.

     I, too, would be interested in other investigators' experience in this
area.

Joel A. Huberman
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